This page contains a link to the B. burgdorferi 5’ end transcriptome and instructions for navigating the dataset. Transcriptional start sites (TSSs) and processed 5’ ends were distinguished with differential application of tobacco acid pyrophosphatase (TAP) in the RNA-seq library preparations. These data will allow users to accurately determine gene boundaries, identify putative leaderless and long untranslated regions, and discover novel transcripts. For specific examples, experimental validation of the data and more information on global analysis of the B. burgdorferi 5’ end transcriptome please refer to our complete publication.
Data are displayed using JBrowse, developed by Skinner at al., 2009. (Skinner at al., 2009. JBrowse: A next-generation genome browser. Genome Research. 19:1630-1638 doi:10.1101/gr.094607.109). More information concerning JBrowse can be found here: http://jbrowse.org
Please cite our Nucleic Acids Research Article if you use this information for publications.
Philip P. Adams, Carlos Flores Avile, Niko Popitsch, Ivana Bilusic, Renée Schroeder, Meghan Lybecker, and Mollie W. Jewett. 2017. In vivo expression technology and 5΄ end mapping of the Borrelia burgdorferi transcriptome identify novel RNAs expressed during mammalian infection. Nucleic Acids Res. 45 (2): 775-792. doi: 10.1093/nar/gkw1180.
We acknowledge the following contributors to the development of the Jewett Lab Interactive Genomics Portal: Cesar A. Rojas, Philip P. Adams and the UCF College of Medicine Health and Information Technology team Aaron Spies, Andres Calvete, Micah Marshall and Craig Anderson.
We acknowledge support from: the National Institute of Allergy and Infectious Diseases of the National Institutes of Health [R01AI099094]; the National Research Fund for Tick-Borne Diseases; the University of Central Florida College of Medicine; the Austrian Science Fund [FWF F4301 and F4308]; and the University of Vienna.